5B2P
Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.611, 159.273, 96.674 |
Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
Resolution | 46.259 - 1.700 |
R-factor | 0.1813 |
Rwork | 0.180 |
R-free | 0.20320 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.391 |
Data scaling software | Aimless |
Refinement software | PHENIX (1.10_2155: ???) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.260 |
High resolution limit [Å] | 1.700 |
Number of reflections | 255229 |
<I/σ(I)> | 19.6 |
Completeness [%] | 98.8 |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 9-11% PEG 3350, 0.2 M calcium acetate, 0.1 M sodium acetate |