5B2P
Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.611, 159.273, 96.674 |
| Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
| Resolution | 46.259 - 1.700 |
| R-factor | 0.1813 |
| Rwork | 0.180 |
| R-free | 0.20320 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.10_2155: ???) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.260 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 255229 |
| <I/σ(I)> | 19.6 |
| Completeness [%] | 98.8 |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 9-11% PEG 3350, 0.2 M calcium acetate, 0.1 M sodium acetate |
