5B2M
A crucial role of Cys218 in the stabilization of an unprecedented auto-inhibition form of MAP2K7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 71.603, 71.603, 264.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.920 - 3.060 |
R-factor | 0.29148 |
Rwork | 0.288 |
R-free | 0.36369 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wzu |
RMSD bond length | 0.023 |
RMSD bond angle | 2.431 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.050 |
Number of reflections | 7938 |
<I/σ(I)> | 13.3 |
Completeness [%] | 98.3 |
Redundancy | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 310 | PEG 3350, sodium citrate tribasic, HEPES, AMPPCP, MgCl2 |