5B04

Crystal structure of the eukaryotic translation initiation factor 2B from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	144.495, 209.234, 223.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 2.994
R-factor	0.2247
Rwork	0.222
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.440
Phasing software	XDS
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.290
High resolution limit [Å]	2.990
Number of reflections	136112
<I/σ(I)>	13.3
Completeness [%]	99.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG200MME, sodium chloride, Na/K phosphate