5AZB

Crystal structure of Escherichia coli Lgt in complex with phosphatidylglycerol and the inhibitor palmitic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9200
Spacegroup name	P 21 21 21
Unit cell lengths	50.413, 60.973, 117.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.426 - 1.600
R-factor	0.1813
Rwork	0.179
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.035
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1634))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.067	0.035
Rmeas	0.069	0.037
Rpim	0.020	0.010	0.359
Total number of observations	607413
Number of reflections	49244
<I/σ(I)>	8
Completeness [%]	99.9	99.9	99.1
Redundancy	12.3	12.2	8.6
CC(1/2)		0.999	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris , CaCl2, and PEG2000 MME