5AZ8
Crystal structure of MBP-Tom20 fusion protein tethered with ALDH presequence via a disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.013, 69.013, 212.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.110 - 1.700 |
| R-factor | 0.16347 |
| Rwork | 0.162 |
| R-free | 0.19555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf 2v1t |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 56782 |
| <I/σ(I)> | 22.1 |
| Completeness [%] | 98.4 |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% PEG 8000, 20% Glycerol, 0.04M Potassium thiocyanate |
