5AXI
Crystal structure of Cbl-b TKB domain in complex with Cblin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.144, 94.091, 133.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.050 - 2.500 |
| R-factor | 0.20375 |
| Rwork | 0.199 |
| R-free | 0.26397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ob2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.731 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.143 | 1.069 |
| Number of reflections | 35334 | |
| <I/σ(I)> | 15.3 | 2.1 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 10.7 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | PEG 3350, BIS-TRIS, ammonium acetate |
