5AX8
Recombinant expression, purification and preliminary crystallographic studies of the mature form of human mitochondrial aspartate aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.706, 76.140, 94.247 |
| Unit cell angles | 78.00, 85.65, 78.41 |
Refinement procedure
| Resolution | 48.315 - 2.989 |
| R-factor | 0.274 |
| Rwork | 0.273 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pdb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.989 | 2.989 |
| Number of reflections | 30161 | |
| <I/σ(I)> | 4.3 | 1.9 |
| Completeness [%] | 97.2 | 97.1 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 0.1 M HEPES pH 6.8, 25% (v/v) Jeffamine ED-2001 pH 6.8 |
