5AX1
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.80 angstrom
Replaces: 3WTAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-31 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.990, 101.630, 43.760 |
| Unit cell angles | 90.00, 119.17, 90.00 |
Refinement procedure
| Resolution | 34.826 - 1.803 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.830 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 26610 | |
| <I/σ(I)> | 13.55 | 1.91 |
| Completeness [%] | 99.5 | 96.16 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | 20% PEG400, 0.1M Tris, 0.1M NaCl, 20mM MgCl2 |
