5AVN
The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.082, 98.285, 85.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.030 |
| R-factor | 0.1286 |
| Rwork | 0.127 |
| R-free | 0.14969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o1h |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.037 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.057 | 0.407 |
| Number of reflections | 386570 | |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.1 M Hepes-NaOH (pH 7.5), 1.75 M (NH4)2SO4 |
