5ARJ
crystal structure of porcine RNase 4 D80A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-31 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 30.832, 61.821, 68.887 |
Unit cell angles | 92.04, 100.99, 101.24 |
Refinement procedure
Resolution | 67.430 - 2.900 |
R-factor | 0.20998 |
Rwork | 0.207 |
R-free | 0.28154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5AR6. |
RMSD bond length | 0.009 |
RMSD bond angle | 1.279 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.390 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.140 | 0.200 |
Number of reflections | 7962 | |
<I/σ(I)> | 8 | 2 |
Completeness [%] | 73.5 | 75.5 |
Redundancy | 1.6 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M SODIUM CACODYLATE PH 6.5, 1.0 M SODIUM CITRATE TRIBASIC DIHYDRATE |