5APU
Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b black
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-02 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.200, 37.520, 95.000 |
| Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
| Resolution | 34.890 - 1.350 |
| R-factor | 0.16513 |
| Rwork | 0.163 |
| R-free | 0.19908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.869 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.030 | 0.670 |
| Number of reflections | 39198 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 95.9 | 92.1 |
| Redundancy | 3.72 | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4 M SODIUM MALONATE PH 5.0 |
