5APG
Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from Vulcanisaeta distributa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-06 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.950, 66.970, 106.420 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 39.208 - 1.600 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.130 | 1.500 |
| Number of reflections | 107521 | |
| <I/σ(I)> | 19.73 | 2.15 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.4 | 26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
