5AMH
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Thalidomide, trigonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 51.720, 51.720, 84.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.770 - 1.200 |
| R-factor | 0.12716 |
| Rwork | 0.126 |
| R-free | 0.15737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.398 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 41611 | |
| <I/σ(I)> | 11.85 | 1.78 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.91 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SODIUM ACETATE PH 4.6, 2.2 M CALCIUM CHLORIDE |
