5AJK
Crystal structure of variola virus virulence factor F1L in complex with human Bak BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 124.774, 68.762, 171.598 |
| Unit cell angles | 90.00, 109.43, 90.00 |
Refinement procedure
| Resolution | 39.702 - 2.550 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d2m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 45043 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 1.7 M MGSO4, 0.1 M NA-ACETATE PH 5.2 |
