5AIU

A complex of RNF4-RING domain, Ubc13-Ub (isopeptide crosslink)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-24
Detector	RIGAKU SATURN
Spacegroup name	P 21 21 2
Unit cell lengths	69.190, 169.210, 52.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.020 - 2.210
R-factor	0.21857
Rwork	0.217
R-free	0.25541
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.696
Data reduction software	xia2
Data scaling software	xia2
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.200	2.260
High resolution limit [Å]	2.210	2.210
Rmerge	0.060	0.150
Number of reflections	29485
<I/σ(I)>	20.9	7.8
Completeness [%]	95.0	74.8
Redundancy	5.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1,2-ETHANEDIOL