5AHJ
Yeast 20S proteasome in complex with Macyranone A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.670, 300.700, 143.990 |
| Unit cell angles | 90.00, 112.52, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.19531 |
| Rwork | 0.194 |
| R-free | 0.21894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENRTY 1RYP |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 250454 | |
| <I/σ(I)> | 14.6 | 2.7 |
| Completeness [%] | 97.4 | 97 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 100 MM MES, PH 7.0 12% MPD 20 MM MGAC2 |
