5AH4
The sliding clamp of Mycobacterium smegmatis in complex with a natural product.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.888, 96.124, 92.272 |
| Unit cell angles | 90.00, 124.41, 90.00 |
Refinement procedure
| Resolution | 76.271 - 2.313 |
| R-factor | 0.1755 |
| Rwork | 0.173 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p16 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.270 | 2.320 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.070 | 0.860 |
| Number of reflections | 47835 | |
| <I/σ(I)> | 19.5 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM MGCL2 16.3 % (V/V) GLYCEROL 12.2 % (W/V) PEG 3350 100 MM TRIS-HCL PH 7.44 |
