5AES
Crystal Structure of murine Chronophin (Pyridoxal Phosphate Phosphatase) in Complex with a PNP-derived Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-02 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 166.810, 166.810, 166.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.714 - 2.751 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bx3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.932 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.710 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.950 |
| Number of reflections | 20219 | |
| <I/σ(I)> | 13 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M IMIDAZOLE (PH 8.0), 0.2M NACL, 1M SODIUM TARTRATE |
