5AEJ
Crystal structure of human Gremlin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-26 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.816, 106.135, 78.521 |
| Unit cell angles | 90.00, 121.25, 90.00 |
Refinement procedure
| Resolution | 41.631 - 1.904 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jph |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.630 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.500 |
| Number of reflections | 47349 | |
| <I/σ(I)> | 15.2 | 2.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2.1 M NACL, 0.1 M NA ACETATE, 0.3 M LI SULFATE, PH 4.5 |
