5AEE
A bacterial protein structure in glycoside hydrolase family 31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.960, 107.620, 103.300 |
| Unit cell angles | 90.00, 107.85, 90.00 |
Refinement procedure
| Resolution | 53.810 - 1.850 |
| R-factor | 0.16446 |
| Rwork | 0.163 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aed |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.870 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.810 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.880 |
| Number of reflections | 121021 | |
| <I/σ(I)> | 13.5 | 1.2 |
| Completeness [%] | 88.0 | 72.9 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 25 MG/ML-1 PROTEIN IN 50 MM NAPO4 , PH6.5, AND 250 MM NACL BUFFER IS MIXED WITH EQUAL VOLUME OF PRECIPITANT COMPOSED OF 50-60% (V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1-0.15 M CACL2, AND 20 DEGREE |
