5ABU

Complex of D. melanogaster eIF4E with the 4E-binding protein Mextli and cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	44.880, 56.400, 99.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.882 - 2.160
R-factor	0.197
Rwork	0.195
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UE8 CHAIN A
RMSD bond length	0.002
RMSD bond angle	0.603
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.220
High resolution limit [Å]	2.160	2.160
Number of reflections	14107
<I/σ(I)>	9	2.2
Completeness [%]	99.3	99.3
Redundancy	6.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4		0.1M MMT (MALIC ACID:MES:TRIS), PH=4.0, 23% PEG1500