5AAM
Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency
Replaces: 4UD3Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.638, 55.891, 87.047 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.490 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.630 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.080 | 1.150 |
| Number of reflections | 23183 | |
| <I/σ(I)> | 14.8 | 1.4 |
| Completeness [%] | 98.3 | 92.3 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
