5AA1

Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	65.853, 135.752, 138.030
Unit cell angles	90.00, 92.01, 90.00

Refinement procedure

Resolution	47.249 - 2.890
R-factor	0.1706
Rwork	0.168
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a5x
RMSD bond length	0.009
RMSD bond angle	1.351
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.250	2.890
High resolution limit [Å]	2.890	2.800
Rmerge	0.120	0.950
Number of reflections	52009
<I/σ(I)>	9.1	1.6
Completeness [%]	92.3	100
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1