5A7I
Crystal structure of INPP5B in complex with biphenyl 3,3',4,4',5,5'- hexakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.975, 96.975, 152.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.290 - 2.890 |
| R-factor | 0.19826 |
| Rwork | 0.197 |
| R-free | 0.22324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n9v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.191 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.290 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.070 | 0.840 |
| Number of reflections | 16922 | |
| <I/σ(I)> | 21.2 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 10% GLYCEROL, 25% PROPANEDIOL AND 0.1 M SODIUM/POTASSIUM PHOSPHATE PH 6.2 |
