5A77

Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 1 21 1
Unit cell lengths	48.573, 95.079, 52.145
Unit cell angles	90.00, 103.71, 90.00

Refinement procedure

Resolution	47.539 - 2.500
R-factor	0.2036
Rwork	0.196
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g9z
RMSD bond length	0.009
RMSD bond angle	1.247
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.540	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.120	0.410
Number of reflections	15872
<I/σ(I)>	7.1	2.7
Completeness [%]	99.2	99
Redundancy	4.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1