5A4D
Crystal structure of the chloroplastic gamma-ketol reductase from Arabidopsis thaliana bound to 13KOTE and NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.160, 128.600, 150.120 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 49.581 - 2.807 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.214 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 2.980 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 75277 | |
| <I/σ(I)> | 13.67 | 2.19 |
| Completeness [%] | 99.4 | 98.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0,2 M SODIUM CHLORIDE, 0,1 M TRIS PH 8,5, 15,5 % PEG 4000 |
