5A19
The structure of MAT2A in complex with PPNP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.220, 95.490, 117.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.710 - 2.340 |
| R-factor | 0.16549 |
| Rwork | 0.163 |
| R-free | 0.20446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p02 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.040 | 2.400 |
| High resolution limit [Å] | 2.330 | 2.340 |
| Rmerge | 0.130 | 0.600 |
| Number of reflections | 16225 | |
| <I/σ(I)> | 20.7 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 30 % PEG 600 |
