5A0X
Substrate peptide-bound structure of metalloprotease Zmp1 variant E143AY178F from Clostridium difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-26 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.435, 42.941, 123.244 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 40.850 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a0p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (in) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.900 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.410 |
| Number of reflections | 43083 | |
| <I/σ(I)> | 9.58 | 2.78 |
| Completeness [%] | 97.8 | 87.8 |
| Redundancy | 6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 293 | 2.25 AMMONIUM PHOSPHATE DIBASIC, 0.1 M TRIS PH 9.0 GROWN AT 293K |
