4ZZH
SIRT1/Activator Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 99.190, 111.640, 132.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.666 - 3.100 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.23810 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.039 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 3.340 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.067 | 0.781 |
| Number of reflections | 13615 | |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Magnesium chloride, 0.1 M Tris pH 8.5, and 16 % w/v PEG 4000 |
