4ZWC
Crystal structure of maltose-bound human GLUT3 in the outward-open conformation at 2.6 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.109, 121.880, 96.078 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 31.700 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zw9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.143 | 0.518 |
| Number of reflections | 49602 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 95.0 | 97.3 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.9 | 293 | 34% PEG 400, 400 mM (NH4)2HPO4, 50 mM Maltose, 100 mM ADA |
