4ZWB
Crystal structure of maltose-bound human GLUT3 in the outward-occluded conformation at 2.4 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.460, 119.487, 53.908 |
| Unit cell angles | 90.00, 103.75, 90.00 |
Refinement procedure
| Resolution | 33.272 - 2.400 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zw9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.086 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.351 |
| Number of reflections | 22422 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 94.6 | 91.4 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 38-40% PEG 400, 100 mM Mg (CHO2)2, 50 mM maltose, 100 mM ADA |
