4ZVE
Crystal structure of GGDEF domain of the E. coli DosC - form I (apo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91162 |
| Spacegroup name | P 41 |
| Unit cell lengths | 70.830, 70.830, 34.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.415 - 1.200 |
| R-factor | 0.1242 |
| Rwork | 0.123 |
| R-free | 0.14550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ign |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.067 | 0.379 |
| Number of reflections | 54503 | |
| <I/σ(I)> | 20.99 | 6.28 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 12.8 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M tri-sodium citrate pH 5.6, 0.2 M ammonium acetate, 30% (w/v) PEG 4000 |
