4ZUR
Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a hydroxamate inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.990, 120.700, 64.520 |
| Unit cell angles | 90.00, 97.04, 90.00 |
Refinement procedure
| Resolution | 22.800 - 1.130 |
| R-factor | 0.115 |
| Rwork | 0.114 |
| R-free | 0.13600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.434 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1833) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 1.190 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.077 | 0.455 |
| Number of reflections | 248014 | |
| <I/σ(I)> | 11.8 | 3.9 |
| Completeness [%] | 98.2 | 94.4 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M NH4Cl, 20% (w/v) PEG 3,350 |
