4ZUN
Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a thiol inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.859, 120.993, 64.710 |
| Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
| Resolution | 41.783 - 1.400 |
| R-factor | 0.1466 |
| Rwork | 0.146 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1833) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.088 | 0.795 |
| Number of reflections | 134698 | |
| <I/σ(I)> | 23.1 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M LiNO3, 20% (w/v) PEG 3,350 |
