4ZU1
Crystal Structure of O-Acetylserine Sulfhydrylase from Haemophilus influenzae in complex with O-acetyl serine and peptide inhibitor
Replaces: 4MILExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.530, 112.530, 43.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.202 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ho1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.777 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 13806 | |
| <I/σ(I)> | 32.9 | 4.71 |
| Completeness [%] | 98.9 | 92 |
| Redundancy | 5.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | o.1 M HEPES 1.2M Sodium Citrate |
