4ZTX
Neurospora crassa cobalamin-independent methionine synthase complexed with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.282963 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.254, 94.630, 114.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.100 |
| R-factor | 0.16599 |
| Rwork | 0.163 |
| R-free | 0.21673 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.007 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 49989 | |
| <I/σ(I)> | 39.7 | 5.8 |
| Completeness [%] | 98.8 | 94.9 |
| Redundancy | 12.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% w/v PEG3350, 150 mM potassium nitrate, 1 mM DTT, 20% v/v glycerol |
