4ZTR
Human Aurora A catalytic domain bound to FK1141
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.270, 83.270, 170.997 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.770 - 2.850 |
| R-factor | 0.21479 |
| Rwork | 0.212 |
| R-free | 0.26781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.059 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.770 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 8800 | |
| <I/σ(I)> | 20.2 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1 M Tris pH 8.0, 28% PEG 4k |
