4ZTQ
Human Aurora A catalytic domain bound to FK932
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.523, 83.523, 168.795 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.400 - 2.800 |
| R-factor | 0.20348 |
| Rwork | 0.201 |
| R-free | 0.24624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.062 | 0.690 |
| Number of reflections | 9189 | |
| <I/σ(I)> | 25.6 | 3.7 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.3 M Ammonium citrate dibasic, 25% PEG 3350 |
