4ZTD
Crystal Structure of Human PCNA in complex with a TRAIP peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.360, 84.360, 210.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.749 - 2.199 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d2g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.749 | 2.252 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.064 | 0.715 |
| Number of reflections | 42654 | |
| <I/σ(I)> | 22.17 | |
| Completeness [%] | 99.2 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 16% PEG 6K in 0.1mM magnesium acetate, MES buffer pH 6.5 |
