4ZS2
Structural complex of FTO/fluorescein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-12 |
| Detector | Bruker AXIOM 200 |
| Wavelength(s) | 0.9735 |
| Spacegroup name | H 3 |
| Unit cell lengths | 142.660, 142.660, 83.218 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.970 - 2.160 |
| R-factor | 0.19921 |
| Rwork | 0.198 |
| R-free | 0.22854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 33566 |
| <I/σ(I)> | 23.2 |
| Completeness [%] | 99.8 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate (pH 5.8), 10% PEG 3350, 8% isopropanol |
