4ZRQ

E88 deletion mutant of CD320 in complex with TC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-07-16
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	98.404, 98.404, 356.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.434 - 2.600
R-factor	0.2001
Rwork	0.199
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.886
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.434
High resolution limit [Å]	2.600	2.600
Rmerge		1.620
Number of reflections	54979
<I/σ(I)>	16.8	2.2
Completeness [%]	99.9	100
Redundancy	10.8	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	PEG3350, L-Proline, Hepes