4ZR0
Full length scs7p (only hydroxylase domain visible)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.74 |
Spacegroup name | I 21 3 |
Unit cell lengths | 202.943, 202.943, 202.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.834 - 3.800 |
R-factor | 0.2473 |
Rwork | 0.246 |
R-free | 0.27070 |
Structure solution method | SIRAS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.723 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.122 | 1.592 |
Number of reflections | 15000 | |
<I/σ(I)> | 8.5 | 1.1 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 287 | 20% PEG3350, 0.1M Hepes, pH7.0, 0.3M (NH4)2SO4 |