4ZR0
Full length scs7p (only hydroxylase domain visible)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.74 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 202.943, 202.943, 202.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.834 - 3.800 |
| R-factor | 0.2473 |
| Rwork | 0.246 |
| R-free | 0.27070 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.723 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.122 | 1.592 |
| Number of reflections | 15000 | |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 287 | 20% PEG3350, 0.1M Hepes, pH7.0, 0.3M (NH4)2SO4 |
