4ZQ9
X-ray structure of AAV-2 OBD bound to AAVS1 site 3:1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.097, 137.255, 79.249 |
| Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.2059 |
| Rwork | 0.205 |
| R-free | 0.22950 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 105074 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 99.1 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 273 | PEG 6K, Sodium Citrate, Lithium Chloride |
