4ZPV
Structure of MERS-Coronavirus Spike Receptor-binding Domain (England1 Strain) in Complex with Vaccine-Elicited Murine Neutralizing Antibody D12 (Crystal Form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.181, 106.145, 171.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.547 - 3.200 |
R-factor | 0.2259 |
Rwork | 0.223 |
R-free | 0.28120 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.547 | 3.360 |
High resolution limit [Å] | 3.200 | 3.250 |
Rmerge | 0.759 | |
Number of reflections | 19408 | |
<I/σ(I)> | 32.4 | 2 |
Completeness [%] | 85.4 | 76.3 |
Redundancy | 5.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 80 mM magnesium acetate, 14.5 % PEG 8,000 |