4ZPN
Crystal Structure of Protocadherin Gamma C5 EC1-3 with extended N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.027, 108.874, 86.612 |
| Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
| Resolution | 66.921 - 3.300 |
| R-factor | 0.2132 |
| Rwork | 0.211 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pzo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.718 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.870 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 13905 | |
| <I/σ(I)> | 9.6 | 3 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 8% (w/v) PEG3350, 200mM potassium nitrate, 3% (v/v) glycerol |
