4ZP6

Coxsackievirus B3 Polymerase - F364A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.233, 74.233, 288.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.797 - 1.648
R-factor	0.1897
Rwork	0.189
R-free	0.22000
RMSD bond length	0.007
RMSD bond angle	0.870
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.797	1.750
High resolution limit [Å]	1.648	1.650
Rmerge	0.048	0.800
Number of reflections	154888
<I/σ(I)>	23.09	0.75
Completeness [%]	83.2	41.8
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.085 M Tris pH 7.5, 1.2 M Ammonium Sulfate , 25% Glycerol