4ZO2

AidC, a Dizinc Quorum-Quenching Lactonase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2015-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.257, 97.880, 110.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.124 - 1.090
R-factor	0.1311
Rwork	0.131
R-free	0.16200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4le6
RMSD bond length	0.015
RMSD bond angle	1.401
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.124	1.110
High resolution limit [Å]	1.090	1.090
Rmerge		0.608
Number of reflections	239792
<I/σ(I)>	7	1.17
Completeness [%]	99.6	99.2
Redundancy	6.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	MgCl2.6H2O (0.2 M), Bis-Tris (0.1 M), pH 6.5, 25 % (w/v) PEG 3350