4ZNC

Fc fragment of human IgG in complex with the C domain of staphylococcal protein A mutant - Q9W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-27
Detector	MAR CCD 130 mm
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	137.951, 87.210, 103.248
Unit cell angles	90.00, 91.06, 90.00

Refinement procedure

Resolution	34.819 - 2.280
R-factor	0.1979
Rwork	0.196
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zmd 4wwi
RMSD bond length	0.016
RMSD bond angle	1.506
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1664)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.137
Number of reflections	52943
<I/σ(I)>	19.9	1.6
Completeness [%]	100.0	99.8
Redundancy	7.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	PEG 5000 MME, ammonium sulfate, sodium acetate