4ZMF
Phosphorylated Aspartate in the Crystal Structure of the Alpha-kinase domain of Myosin-II Heavy Chain Kinase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.098, 109.960, 79.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.060 - 2.390 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lkm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 29130 | |
| <I/σ(I)> | 27.04 | 4.66 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.5 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | PEG 8000, sodium phosphate, Tris chloride |
