4ZLT
Crystal structure of viral chemokine binding protein R17 in complex with CCL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-13 |
Detector | NOIR-1 |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 98.493, 109.482, 210.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.247 - 3.000 |
R-factor | 0.2195 |
Rwork | 0.215 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ZKQ and 2X6G |
RMSD bond length | 0.003 |
RMSD bond angle | 0.462 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 49.200 | 3.680 |
High resolution limit [Å] | 2.761 | 19.680 | 2.780 |
Number of reflections | 26825 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 100.0 | ||
Redundancy | 4.7 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 15-20% PEG 3350 0.2-0.4M MgFormate |